Tsb medium caracteristicas generales11/11/2023 ![]() ![]() PREPARATION OF MACCONKEY AGAR MEDIUM (MAC) IN LABORATORY can grow in the low oxygen environment.Ĭheck out the preparation of MacConkey Agar medium in Laboratory grow best in the presence of oxygen and it is also a Facultative anaerobic organism i.e. ⇒ Oxygen requirements – Klebsiella pneumoniae (K. Also, the pH requirement varies as per the strain of Klebsiella pneumoniae. pneumoniae can survive alkaline pH but not at acidic pH and the maximum growth of the organism is observed at 7.0 i.e. ⇒ Optimum temperature – Klebsiella pneumoniae ranges from 15–40☌ but the optimum temperature for most of the strains is 37☌ and usually cultivated at this temperature in laboratories. Commonly the NAM & MacConkey Agar medium is used for the cultivation of Klebsiella pneumoniae in the Laboratory. ⇒ Special requirements – Klebsiella pneumoniae has no complex nutritional requirements and readily grows in ordinary media like Nutrient Agar medium (NAM). Gram Staining Reaction – Klebsiella pneumoniae is a Gram -ve (Negative) bacterium.ĬULTURE REQUIREMENTS OF KLEBSIELLA PNEUMONIAE (K. Spores – The Klebsiella pneumoniae is a non–sporing bacterium.Ĭapsule – Capsules are present in Klebsiella pneumoniae which can easily be demonstrated using India ink preparation, appear as a clear halo in a dark background. ![]() pneumoniae is a non-flagellated bacterium. Motility – Klebsiella pneumoniae is a non-motile bacterium.įlagella – K. pneumoniae is arranged singly, in pairs, or in short chains and sometimes in clusters. Size – The size of Klebsiella pneumoniae is about 1–2 µm × 0.5–0.8 µm (micrometer).Īrrangement Of Cells – K. Shape – Klebsiella pneumoniae is a short, plump, straight rod shape (bacillus) bacterium. That’s all about the Morphology and Culture Characteristics of Klebsiella pneumoniae…. ![]()
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